Overview
Grapevine viruses
Our ELISA tests for grape samples
Group A
ArMV Arabis mosaic virus (samples: leaves*, wood***)
GFLV Grapevine fanleaf virus (samples: leaves*, wood***)
ArMV+GFLV Arabis mosaic virus + Grapevine fanleaf virus (samples: leaves*, wood***)
GPGV Grapevine pinot gris virus (samples: leaves*, wood***)
RpRSV-ch Raspberry ringspot virus-ch (samples: leaves*, wood***)
RpRSV-g Raspberry ringspot virus-g (samples: leaves*, wood***)
TBRV Tomato black ring virus (samples: leaves*, wood***)
TRSV Tobacco ringspot virus (samples: leaves*, wood***)
ToRSV Tomato ringspot virus (samples: leaves*, wood***)
ToRSV-Ch Tomato ringspot virus-Ch (samples: leaves*, wood***)
SLRSV Strawberry latent ringspot virus (samples: leaves*, wood***)
Group B
GLRaV-1 Grapevine leafroll-assoc. virus 1 (samples: leaves*, petioles**, wood***)
GLRaV-1+3 Grapevine leafroll-assoc. virus 1+3 (samples: leaves*, petioles**, wood***)
GLRaV-2 Grapevine leafroll-assoc. virus 2 (samples: leaves*, petioles**, wood***)
GLRaV-3 Grapevine leafroll-assoc. virus 3 (samples: leaves*, petioles**, wood***)
GLRaV-4 strain 6 Grapevine leafroll-assoc. virus 4 strain 6 (samples: leaves*, petioles**, wood***)
GLRaV-4 strains Grapevine leafroll-assoc. virus generic 4 strains (samples: leaves*, petioles**, wood***)
GVA Grapevine virus A (samples: leaves*, petioles**, wood***)
GFkV Grapevine fleck virus (samples: leaves*, petioles**, wood***)
*Leaves from young shoots at the beginning of the growing season.
**Well-developed mature leaves, especially petioles and leaf veins, from the lower part of the plant towards the end of the growing season.
***Phloem tissue from mature canes during dormancy.
Our PCR tests for grapevine samples
GRBV Grapevine red blotch virus (samples: leaves*, petioles**, wood***)
GVA**** Grapevine virus A (samples: leaves*, petioles**, wood***)
GVB Grapevine virus B (samples: leaves*, petioles**, wood***)
RSPaV**** Rupestris stem pitting assoc. virus (samples: leaves*, petioles**, wood***)
FD Flavescence dorée phytoplasma (samples: leaves*, petioles**, wood***)
BN Bois noir phytoplasma (samples: leaves*, petioles**)
*Leaves from young shoots at the beginning of the growing season.
**Well-developed mature leaves, especially petioles and leaf veins, from the lower part of the plant towards the end of the growing season.
***Phloem tissue from mature wood during dormancy.
****PCR tests for GVA and RSPaV are not part of the accredited service.
Our recommendations for sampling
Virus tests
We use efficient and reliable techniques to detect viruses in the vine. However, the virus concentration varies considerably depending on the tissue source, virus and physiological state of the host, as well as the time of year. This must be taken into account to obtain reliable results.
In principle, all viruses can be detected in mature canes during dormancy. However, when examining leaves, the viruses are divided into two different groups, each of which has specific requirements. Under the climatic conditions of Switzerland and Central Europe, the following recommendations can be made for the collection of tissue samples:
Virus group A test: preferably tissue samples
Leaves from young shoots and juicy bark at the beginning of the growing season and bark sections (phloem) from mature canes during dormancy.
Virus group A test: Unsuitable tissue samples
Samples collected during the hot summer or old leaves in the fall.
Virus group B test: preferably tissue samples
Well-developed mature leaves, especially petioles and leaf veins, from the lower part of the plant towards the end of the season as well as bark sections (phloem) from mature canes during dormancy.
Virus group B test: Unsuitable tissue samples
Young tissue at the beginning of the growing season.
For testing rootstocks, wood from mature canes is the only reliable sample tissue.
Phytoplasma tests
Phytoplasmas (Flavescence dorée, Bois noir) are best detected in the veins and petioles of well-developed, mature leaves towards the end of the growing season, as are group B viruses. During the rest of the year, phytoplasmas are preferably detected in the root tissue.
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